Sonication:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
DNA Library Preparation:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
Polymerase Chain Reaction:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
Purification:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
Activation Assay:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
cDNA Synthesis:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
Real-time Polymerase Chain Reaction:Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Sonicated Salmon Sperm Agilent technologies Cat# 201190 Lyse/Fix buffer (5X) BD Biosciences Cat# 558049 Perm Buffer III BD Biosciences Cat# 558050 FOXP3 Fix/Perm Buffer (4x) BioLegend Cat# 421401 FOXP3 Perm Buffer (10x) BioLegend Cat# 421402 Hexadecyltrimethylammonium bromide MilliporeSigma Cat# H5882 Dianisidine Dihydrochloride MilliporeSigma Cat# D3252 Mouse IL1b PeproTech Cat# 211-11B Mouse IL2 R&D Systems Cat# 402-ML Mouse IL4 R&D Systems Cat# 404-ML Mouse IL5 PeproTech Cat# 215-15 Mouse IL6 PeproTech Cat# 216-16 Mouse IL10 PeproTech Cat# 210-10 Mouse IL12 R&D Systems Cat# 419-ML Mouse IL13 PeproTech Cat# 210-13 Mouse IL17 PeproTech Cat# 210-17 Mouse IL23 BioLegend Cat# 589002 Mouse IFNg R&D Systems Cat# 485-MI Mouse TGFb BioLegend Cat# 763102 Mouse CCL21 R&D Systems Cat# 457-6C Mouse CCL5 BioLegend Cat# 594202 Mouse CXCL12 BioLegend Cat# 578702 Mouse Lipocalin-2 R&D Systems Cat# 1857-LC Human IL1b PeproTech Cat# 200-01B Human IL2 R&D Systems Cat# 202-IL Human IL4 R&D Systems Cat# 204-IL Human IL5 R&D Systems Cat# 205-IL Human IL6 R&D Systems Cat# 206-IL Human IL10 PeproTech Cat# 200-10 Human IL12 R&D Systems Cat# 219-IL Human IL13 R&D Systems Cat# 213-ILB Human IL17A R&D Systems Cat# 317-ILB Human IL23 R&D Systems Cat# 1290-IL Human IFNg R&D Systems Cat# 285-IF Human TGFb R&D Systems Cat# 240B-002 Human CCL21 BioLegend Cat# 582204 Human CCL5 PeproTech Cat# AF-300-06 Human CXCL12 BioLegend Cat# 581202 Critical Commercial Assays Illumina Tagment DNA TDE1 Enzyme and Buffer Illumina Cat# 20034197 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1001 QIAprep Spin Miniprep Kit QIAGEN Cat# 27106 MinElute PCR Purification Kit QIAGEN Cat# 28004 Rac Activation Assay Cell Biolabs Cat# STA-401-2 TaqMan Genotyping Master Mix Applied Biosystems Cat# 4371355 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 2X All-in-One qPCR Mix GeneCopoeia Cat# QP001 (Continued on next page) e4 Cell Reports 31, 107820, June 30, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data ATAC-seq This paper GEO: GSE148672 Oligonucleotides See Table S1 for qPCR primers N/A N/A Software FlowJo FlowJo https://www.flowjo.com/ Other Protein G Agarose MilliporeSigma Cat# 16-266 Protein A Agarose MilliporeSigma Cat# 16-125 6.5 mm Transwell, 5.0 mm Pore Insert Corning Cat# 3421 LS column Miltenyi Biotec Cat# 130-042-401 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD8a (Ly-2) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-044 CD4 MicroBeads, human Miltenyi Biotec Cat# 130-045-101 CD8 MicroBeads, human Miltenyi Biotec Cat# 130-045-201 CD14 MicroBeads, human Miltenyi Biotec Cat# 130-050-201 CD45RO MicroBeads, human Miltenyi Biotec Cat# 130-046-001 Anti-PE MicroBeads Miltenyi Biotec Cat# 130-048-801
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